Gene Knockout Protocols true
By:Martin J. Tymms,Ismail Kola
Published on 2001 by Springer Science & Business Media
As the major task of sequencing the human genome nears completion and the full complement of human genes are catalogued, the task of understanding the normal biological functions of genes and how their alteration leads to diseased states becomes more imperative. In Gene Knockout Protocols, highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for genetic phenotype analysis. These proven methods contain step-by-step instructions, as well as notes on pitfalls to avoid, and emphasize techniques that are relevant to researchers carrying out gene targeting work. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. Issues such as the use of existing mouse mutation resources and the influence of genetic background and epigenetic effects upon phenotype are also covered. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.
This Book was ranked at 29 by Google Books for keyword Protocols & APIs.
Book ID of Gene Knockout Protocols's Books is jZpdJukfew0C, Book which was written byMartin J. Tymms,Ismail Kolahave ETAG "Zr1FBkoFYgc"
Book which was published by Springer Science & Business Media since 2001 have ISBNs, ISBN 13 Code is 9781592592203 and ISBN 10 Code is 1592592201
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Book which have "431 Pages" is Printed at BOOK under CategoryScience
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