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Download PCR Cloning Protocols Pdf

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By:Bing-Yuan Chen,Harry W. Janes
Published on 2002 by Springer Science & Business Media


Annotation PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

This Book was ranked at 26 by Google Books for keyword Protocols & APIs.

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Book which was published by Springer Science & Business Media since 2002 have ISBNs, ISBN 13 Code is 9781592591770 and ISBN 10 Code is 1592591779

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